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METHOD FOR IDENTIFYING THE MAMMALIAN ANIMAL SPECIES AT THE ORIGIN OF PROTEINS CONTAINED IN A SAMPLE BEING TESTED

机译:在样品中鉴定蛋白质来源的哺乳动物动物物种的方法

摘要

The present invention relates to a method for identifying the mammalian animal species at the possible origin of the proteins contained in a sample of biological material being tested, characterized in that the following steps are carried out, wherein: a) a purified peptide preparation is made from an extract of the total proteins of said sample, and b) a spectrum of said preparation is produced by so-called MALDI-TOF MS mass spectrometry, and c) the spectrum obtained in step b) is compared to previously produced reference spectra of various peptide extracts of dental pulp samples from various mammalian animal species, and d) a mammalian animal species at the origin of said tested specimen is identified as being that of said reference mammalian specie(s), if all of the peaks of said reference spectrum of step c) above are found in the spectrum obtained in step b) above.
机译:本发明涉及一种在待测生物材料样品中所含蛋白质的可能来源中鉴定哺乳动物动物物种的方法,其特征在于进行以下步骤,其中:a)制备纯化的肽制剂从所述样品的总蛋白质的提取物中提取,并且b)通过所谓的MALDI-TOF MS质谱法产生所述制剂的光谱,并且c)将步骤b)中获得的光谱与先前产生的参考光谱进行比较。来自各种哺乳动物的牙髓样品的各种肽提取物,和d)如果所述参考光谱的所有峰均被鉴定为所述参考哺乳动物的物种,则位于所述测试样品的起源处的哺乳动物动物在以上步骤b)中获得的光谱中发现了以上步骤c)的结果。

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