METHOD OF STRENGTHENING SEED DORMANCY OF WHEAT BY ACCUMULATING GENE MUTATIONS ENCODING ABSCISIC ACID METABOLIZING ENZYMES

摘要

A method of detecting loss-of-function mutations for the gene encoding abscisic acid 8'-hydroxylase of test wheat, including detecting insertion mutations in the gene encoding abscisic acid 8'-hydroxylase of the wheat D genome by performing nucleic acid amplification using one or more oligonucleotide primers containing a specific base sequence corresponding to the insertion sequence discovered in the abscisic acid 8'-hydroxylase of the wheat D genome, or a base sequence of at least 15 consecutive bases of a complementary sequence thereof, for genome DNA derived from test wheat.