A method for enhancing seed dormancy of wheat by accumulation of abscisic acid metabolizing enzyme gene mutations

摘要

For genomic DNA of the test wheat derived from the nucleotide sequence of at least 15 bases of contiguous or its complementary sequence specific nucleotide sequence corresponding to the insertion sequence found in the abscisic acid 8 'position hydroxylase gene of the wheat D genome By performing nucleic acid amplification using oligonucleotide primers of one or more, including, 'comprising detecting an insertion mutation in position hydroxylase gene, abscisic acid 8 of the test wheat' abscisic acid 8 of the wheat D genome position a method for detecting a loss-of-function mutations in the hydroxylase gene.