Method for measuring fluorescent light amount or light absorption amount and device for measuring the same

摘要

An amount of fluorescent light (5) generated from the specimen (4) is quantitatively measured as "a light-amount value per unit area traceable to the national standard" instead of in terms of a ratio (a ratio value) as in the past. A method for measuring a fluorescent light amount, whereby a specimen (4) is irradiated with excitation light (2), and fluorescent light (5) generated from the specimen (4) is measured by a light-receiving element via a receiving optical system (6), said method comprising the steps of irradiating the specimen (4) with excitation light (2) having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen (4) beforehand, causing the light-receiving element to measure the fluorescent light (5) generated from the specimen (4) via the receiving optical system (6), and computing a fluorescent light-amount as measured by the light-receiving element via the receiving optical system (6) on the basis of an excitation light-amount per the specified unit area, optical coefficients of the receiving optical system (6), and the acceptance coefficient of the light-receiving element, thereby working out a light amount value per the unit area traceable to the national standard.