USE OF A CULTURE MEDIUM CONDITIONED BY MESENCHYMAL STEM CELLS FOR DIFFERENTIATION OF PLURIPOTENT HUMAN STEM CELLS

摘要

The present invention relates to the use of a culture medium conditioned by mesenchymal stem cells (MSC) from adipose tissue for the differentiation of pluripotent stem cells (PSC) into hematopoietic cells as well as other mesodermal cells. The present invention likewise relates to a method for differentiating said PSC that includes: culturing PSC in the culture medium conditioned by the MSC from adipose tissue, such as to obtain 70 to 100% confluence in the culture; preparing embryoid bodies (EB); and treating the EBs with at least one agent that induces cell differentiation.