METHOD FOR CONTROLLED INDUCTION OF SOMATIC MUTATIONS AND USE THEREOF IN PROTEOMICS

摘要

is The present invention, (1) in the medium and culture conditions suitable for the cells to be mutated, wherein at least -IgM , anti -CD19 and / or wherein the addition of the complex to the cell while -DC21 be variations (2) comprising screen to screen the anti-biotin antibody and -IgM set specifically by streptavidin coupled to a support to a method of inducing somatic mutations in vitro or ex vivo. Glass has decided, DNA polymerase inhibiting pol (iota) activates and / or to which the variations are controlled by stimuli / or are adjustable. The invention is particularly applicable to the rapid and controlled variations induced lymphoma BL2 bucket.