CANINE AMNIOTIC FLUID-DERIVED MESENCHYMAL STEM CELL AND A CELL THERAPEUTIC AGENT CONTAINING THE SAME

摘要

PURPOSE: A cell therapeutic agent containing amniotic fluid-derived mesenchymal stem cells is provided to ensure superior differentiation ability.;CONSTITUTION: A method for preparing canine amniotic fluid-derived mesenchymal stem cells comprises: a step of collecting amniotic fluid from a pregnant dog using a syringe; a step of centrifuging amniotic fluid to collect pellet; a step of culturing the pellet in a medium containing bFGF(basic fibroblast growth factor) and EGF(epidermal growth factor) and collecting cultured cells; and a step of isolating and purifying the stem cells. The method for further comprises a step of inducing cell adhesion. The medium contains 0.5-20 ng of bFGF, 1-40 ng of EGF, 1-40% FBS(fetal bovine serum), and 0.5-4% penicillin-streptomycin. A composition for controlling differentiation of mesenchymal stem cells to adipocytes contains FBS, IBMX, dexamethasone, indomethacin, and insulin as active ingredient.;COPYRIGHT KIPO 2011