DUPLEX VIBRIOCIDAL ASSAY FOR SIMULTANEOUSLY DETERMINING VIBRIOCIDAL TITERS OF MIXED VIBRIO VACCINES

摘要

PURPOSE: A duplex vibriocidal assay for simultaneously determining vibriocidal titer is provided to reduce analysis time, labor, and reagent amount. CONSTITUTION: A duplex vibriocidal assay comprises: a step of inoculating mixed vibrio vaccine to human body or animal to obtain serum from a vaccinated person; a step of inactivating complement from the serum and diluting; a step of mixing the serum, vibrio strain, and complement to prepare a mixture; a step of inoculating the mixture to a medium and culturing; a step of measuring vibriocidal titer. The vibrio strain is V. parahaemolyticus, Non-O1 V. cholerae, V. vulnificus, or V. cholerae such as V. cholerae O1 Inaba, V. cholerae O1 Ogawa, or V. cholerae O139.