PRIMERS AND ITS APPLICATION IN MULTIPLEX PCR TO IDENTIFY EASTERN EQUINE ENCEPHALITIS, WESTERN EQUINE ENCEPHALITIS, AND VENEZUELAN EQUINE ENCEPHALITIS VIRUSES

摘要

PURPOSE: single pipe multiplex RT -PCR detects eastern equine encephalitis simultaneously, Western equine encephalitis, the building of venezuelan equine encephalitis virus quick and efficient be provided to viral diagnosis system. ;CONSTITUTION: single pipe multiplex RT -PCR detects eastern equine encephalitis, Western equine encephalitis simultaneously, and the RT-PCR of venezuelan equine encephalitis virus includes primer set, reaction buffer. Archaeal dna polymerase, conversely reverse record enzyme. A kind of primer RT-PCR primer set includes sequence number 1 and 2, sequence number 3 and 4 or sequence number 5 and 6. ;The 2011 of copyright KIPO submissions