METHOD FOR MANUFACTURING BISPECIFIC ANTIBODY OF A TWO-IN-ONE TYPE

摘要

PURPOSE: A method for manufacturing bispecific antibody of a two-in-one type is provided to select phage which is able to recognize two different antigens. ;CONSTITUTION: A method for manufacturing bispecific antibody of a two-in-one type comprises: a step of selectively enriching a phage group having a specific antibody fragment and collecting the phages; a step of infecting the phage to a host cell to prepare a plasmid; a step of removing DNA fragment of light chain from the plasmid and inserting a light chain library to prepare a light chain shuffling library; and a step of panning the light chain shuffling library with a second target antigen. The host cell is E. coli.;COPYRIGHT KIPO 2011