THE METHOD FOR DETECTION OF BISPHENOL A USING GENOMIC ALTERATION OF BISPHENOL A TREATMENT IN THE TESTIS

摘要

PURPOSE: A method for detecting bisphenol A using reproductive system chromosome aberration is provided to determine bisphenol A presence in a test sample. CONSTITUTION: A method for detecting bisphenol A(BPA) of genetic toxic level in a test sample comprises: a step of isolating genomic DNA from reproductive cells of a male experimental animal except for human body; a step of measuring copy number from the genomic DNA; and a step of confirming copy number variation(CNV) in the genome. The reproductive cell is derived from testis or epididymis. The animal is rat, mouse, rabbit, guinea pig, hamster, dog, or cat. In case of a rat or mouse, the CNV exists in Chr2:77739470-77848433, Chr5:128329169-128329869, Chr7:7312289-10272836, Chr9:101951419-101951819, Chr10:72178606-72179006, Chr12:17803411-25366481, or Chr14:4551029-10397399. A gene relating to pheromone reaction is Vomeronasal 1 receptor, J2, E11, L1, G2, G3, G4, G5, G6, G7, G11, or K1.