MICROALAGE TRANSFORMED BY A VECTOR WHICH IS USEFUL FOR MICROALGAE TRANSFORMATION

摘要

PURPOSE: A binary vector is provided to stably express foreign gene induced in microalgae and to easily select transformed microalgae.;CONSTITUTION: A reporter gene for sorting transformed microalgae has optimized 3rd codon in a reporter gene. The GC% of 3rd codon in the reporter gene is 70-90%. The reporter gene is crGUS, crGFP, or crlux. crGUS is denoted by sequence number 1. A binary vector contains the reporter gene. The vector additionally contains pSaD promoter, rbcS2 promoter, pHsp70A promoter, or beta-tublin promoter, and a target gene which is operatively linked to the promoter. The promoter is derived from microalgae. The promoter contains a microalgae-derived intron at the downstream. The target gene is bleomycin resistance gene(ble) or paromomycin resistance gene(part).;COPYRIGHT KIPO 2011