RECOMBINANT E.COLI MK-15 FOR THROMBOLYTIC ENZYME EXPRESSION

摘要

PURPOSE: A recombinant E.coli MK-15 for thrombolytic enzyme expression is provided to ensure ability of preventing blood coagulation and to produce thrombolytic enzyme of high purity.;CONSTITUTION: A recombinant expression vector contains a thrombolytic enzyme gene of sequence number 5. The recombinant expression vector is pCOK-15 recombinant expression vector(KCTC 11666BP). A recombinant E.coli MK-15 is prepared by transforming the recombinant expression vector. The recombinant E.coli MK-15 is cultured in a medium containing soybeans at 35-40°C for 40-55 hours. The medium contains 40-60g of soybeans, 1-10g of tryptone, 0.1-5g of dextrose, 0.5-7g of NaCl, and 0.1-5g of dipotassium phosphate.;COPYRIGHT KIPO 2012