THE METHOD FOR DETECTING RESISTANCE MUTATIONS OF INFLUENZA VIRUS GENE AGAINST ANTI-VIRAL AGENTS USING PYROSEQUENCING

摘要

The present invention provides a kit comprising (i) a primer set of SEQ ID NO: 1 and SEQ ID NO: 2; Primer sets of SEQ ID NO: 3 and SEQ ID NO: 4; Primer sets of SEQ ID NO: 5 and SEQ ID NO: 6; Primer sets of SEQ ID NO: 7 and SEQ ID NO: 8; And performing PCR on the extracted RNA using the primer sets of SEQ ID NO: 9 and SEQ ID NO: 10; And (ii) pyro sequencing the sequencing primers of SEQ ID NOs: 11 to 18 for the PCR product obtained in step (i), a resistance mutation of the gene against the antiviral agent of swine flu virus (H1N1v). It provides a method for detecting. The detection method of the present invention can quickly and accurately detect antiviral gene resistance mutations in the NA gene associated with oseltamivir resistance and the M2 gene associated with amantadine resistance in swine flu present in a respiratory specimen of a patient.