BACILLUS VELEZENSIS A-68-DERIVED CELLULASE GENE AND E.COLI A-68 STRAIN TRANSFORMED BY INTRODUCING THE SAME

摘要

PURPOSE: A gene of Bacillus velezensis-derived cellulase and a recombinant E.coli A-68 recombinant strain transformed by introducing the same are provided to enhance productivity and efficiency of strains.;CONSTITUTION: A Bacillus velezensis A-68(deposit number KACC 91178P)-derived cellulase is encoded by a gene having a sequence of sequence number 1. A recombinant vector for expressing cellulase in E.coli is prepared by linking the gene of sequence number 1 to p-TGEM T-easy vector. The recombinant E.coli A-68(deposit number KACC 91335P) is prepared by transforming through introduction of the recombinant vector pTA-68. A method for producing cellulase comprises a step of culturing the recombinant E.coli A-68 in a medium containing 50g/L of rice bran and 2.5 g/L of yeast extract at pH 7.3 and 35°C.;COPYRIGHT KIPO 2012