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DETECTION CHIP FOR PERFORMING VIRULENCE ANALYSIS OF CLOSTRIDIUM DIFFICILE, DETECTION KIT, AND DETECTION METHOD USING THE SAME
DETECTION CHIP FOR PERFORMING VIRULENCE ANALYSIS OF CLOSTRIDIUM DIFFICILE, DETECTION KIT, AND DETECTION METHOD USING THE SAME
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机译:进行梭菌难度毒力分析的检测芯片,检测工具包以及使用该检测方法的检测方法
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摘要
PURPOSE: A test chip and a kit for analyzing pathogenecity of Clostridium difficile are provided to accurately test risk of Clostridium difficile and quickly block Clostridium difficile infection. CONSTITUTION: A test chip for analyzing pathogenecity of Clostridium difficile contains a probe having oligonucleotides of sequence numbers 36-37 or complementary oligonucleotides thereof. A real-time PCR kit for early analyzing pathogenecity of Clostridium difficile contains a primer pair of sequence numbers 5 and 6 for amplifying toxin B gene, DNA polymerase, dNTPs, PCR buffer solution, and PCR product marker. The marker is labeled at one end of a probe with an oligonucleotide of sequence number 74 or complementary oligonucleotide thereof.
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