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Norovirus standardized positive control and Norovirus detecting kits being provided with RNA transcripts thereof

机译:诺如病毒标准化的阳性对照和诺如病毒检测试剂盒及其RNA转录本

摘要

PURPOSE: A norovirus detection kit having a norovirus positive control and RNA transcript is provided to accurately and quickly detect norovirus and hepatitis A virus. CONSTITUTION: A novovirus positive control group has a sequence of hepatitis A virus VP1/2A gene in a base sequence of capsid gene. The norovirus capsid gene is PCR product amplified by a primer pair for detecting GI group of sequence number 1 and 2, or a primer pair for detecting GII group of sequence number 4 and 5. A detection kit for norovirus contains a primer pair for detecting norovirus GI or GII group having bases of sequence numbers 1 and 2 or 4 and 5, and norovirus positive control group RNA transcript.
机译:目的:提供一种具有诺如病毒阳性对照和RNA转录本的诺如病毒检测试剂盒,可准确,快速地检测诺如病毒和甲型肝炎病毒。组成:新病毒阳性对照组在衣壳基因的碱基序列中具有甲型肝炎病毒VP1 / 2A基因的序列。诺如病毒衣壳基因是通过用于检测序列号1和2的GI组的引物对或用于检测序号4和5的GII组的引物对扩增的PCR产物。诺如病毒的检测试剂盒包含用于检测诺如病毒的引物对具有序列号1和2或4和5的碱基的GI或GII组,以及诺如病毒阳性对照组RNA转录物。

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