METHOD rapid diagnosis of rotavirus infection in the material from the upper respiratory tract, IMMUNOGLOBULIN fluoresce DRY FOR ITS IMPLEMENTATION

摘要

1. A method for rapid diagnosis of rotavirus infections in the materials of the upper respiratory tract, comprising taking a smear, the processing of its phosphate-buffered saline, preparing a suspension by centrifugation of cells, wherein the cells obtained columnar epithelium of the nasal cavity mucous membrane is applied on glass slides and antigen rotavirus in them are determined by the processing of glass fluorescent protivorotavirusnym immunoglobulin and counting fluorescent cells with a fluorescence microscope, and the diagnosis is established with the presence of 3-5 specific luminescence and more infected cells with the virus. ! 2. Fluorescent protivorotavirusny immunoglobulin to determine the rotavirus antigen of claim 1, consisting of fluorestsiinizotiotsianatom conjugated (FITC) protivorotavirusnogo immunoglobulin obtained from the rabbit serum immunized with the rotavirus and having a ratio of FITC: immunoglobulin to 3.5: 1 at a concentration of immunoglobulin in liquid conjugate 1,5 ± 0,5%. ! 3. Fluorescent protivorotavirusny immunoglobulin according to claim 2, characterized in that it further comprises merthiolate preservative at a concentration in the preparation of 1: 10000. ! 4. Fluorescent protivorotavirusny immunoglobulin according to claim 3, characterized in that it is a dry lyophilized powder having a residual moisture content of not more than 2.5%.