APTAMER AGAINST MIDKIN AND ITS APPLICATION

摘要

1. Aptamer having inhibitory activity against midkin. ! 2. The aptamer according to claim 1, in which the aptamer does not have inhibitory activity against pleiotropin. ! 3. The aptamer according to claim 1, having binding activity to the N-terminal fragment of midkin. ! 4. The aptamer according to claim 1, having binding activity to the C-terminal fragment of midkin. ! 5. The aptamer according to claim 2, having binding activity to the N-terminal fragment of midkin. ! 6. The aptamer according to claim 2, having binding activity to the C-terminal fragment of midkin. ! 7. Aptamer, which exhibits inhibitory activity against midkin, inhibiting the binding of midkin and PTPζ. ! 8. The aptamer according to claim 1, which corresponds to either (a) or (b) from the following:! (a) an aptamer comprising a nucleotide sequence selected from SEQ ID NO: 1-70 (with the proviso that uracil may be thymine), wherein the nucleotides contained in the aptamer are such that! (i) at the 2'-positions of the pyrimidine nucleotides, identical or different, are fluorine atoms or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, hydroxyl groups and methoxy groups, and! (ii) at the 2'-positions of the purine nucleotides, identical or different, are hydroxyl groups or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, methoxy groups and fluorine atoms; ! (b) an aptamer comprising a nucleotide sequence selected from SEQ ID NO: 1-70 (with the proviso that uracil may be thymine), wherein one or more nucleotides are substituted, deleted, inserted, or added, and the nucleotides contained in the aptamer are , what ! (i) at the 2'-positions of the pyrimidine nucleotides, identical or different, p