METHOD AND KIT FOR IMMUNE-ENZYME ASSAY OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C2

摘要

FIELD: medicine.;SUBSTANCE: micro-well attachment of the pharmaceutical preparation Derinate with drug substance represented with sodium deoxyribonucleate. Thereafter, an analysed sample containing component C2 of undefined activity and reagent R2 solution (human blood serum with inactivated component C2) is introduced into the wells. It is followed with incubation, and after washing and drying the tray, conjugated enzyme with component C3 antibodies, and its substratum are introduced into the wells. The component C2 activity is calculated by the amount of the enzymatic reaction product. A kit comprises a flat-bottomed micro-tray with attached Derinate, conjugated enzyme with human complement component C3 antibodies, reagent R2 (human blood serum with inactivated component C2), a substratum buffer and a standard with defined C2 activity.;EFFECT: method allows reliable component C2 detection with the use of the available and stable preparation Derinate as an activator.;2 cl, 1 dwg, 1 ex