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METHOD AND KIT FOR IMMUNE-ENZYME ASSAY OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C4
METHOD AND KIT FOR IMMUNE-ENZYME ASSAY OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C4
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机译:人体补体成分C4功能功能免疫酶学测定的方法和工具包
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摘要
FIELD: medicine.;SUBSTANCE: thrombin as a component C4 activator is sorbed in micropanel wells; a sample containing a human complement component C4 of unknown activity is introduced; that is followed by incubation with EDTA added; an enzyme and human anti-complement component C4 antibody conjugate and a substrate thereof are introduced; activity of the component C4 is estimated by the amount of the formed enzymatic reaction product. A kit for assay of functional activity of the human complement component C4 contains a flat-bottomed microplate with sorbed thrombin, the enzyme and human anti-complement component C4 antibody conjugate, the substrate buffer and donor blood serum of known C4 activity as a reference.;EFFECT: group of invention enables estimating functional activity of the component C4 of the human complement system and possesses good reproducibility and no need for activation of the whole complement system.;2 cl, 1 dwg
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