METHOD FOR DETERMINING NEUTROPHIL CAPACITY BY NITROBLUE TETRAZOLIUM REDUCTION REACTION

摘要

FIELD: medicine.;SUBSTANCE: heparinised blood, isotonic solution containing 0.1 % nitroblue tetrazolium are introduced in plate wells. The mixture is incubated for 30 minutes at 37°C, added with 3% acetic acids, heparinised blood preliminary dissolved in isotonic solution of BCG vaccine containing 0.1 % nitroblue tetrazolium. Further, the prepared mixture is incubated for 30 minutes at 37°C, added with 3 % acetic acids. It is followed with counting neutrophils with observed cytoplasm violet-blue formazan granule depositions in a Gorjaev's chamber with taking into account 100-200 neutrophils, and the percentage of positive NBT-cells is assessed.;EFFECT: use of the method enables higher accuracy and reliability of determining neutrophil capacity and reduced acquisition time.;3 tbl, 3 ex