METHOD OF PRODUCING ERYTHROCYTIC DIAGNOSTIC HISTOPLASMOSIS AND COCCIDIODOMYCOSIS ANTIGEN

摘要

FIELD: medicine.;SUBSTANCE: substance of the invention involves H.capsulatum G18 5 P cultivation in a mycelial growth phase on a Smith synthetic protein-free salt medium at temperature 28°C for 3 months then a biomass is separated by bulky filtration and sterilised through membrane filters that is followed by a sterility test, and then the antigens found in the culture medium are precipitated with acetone cooled to -20°C and dried. The erythrocytic diagnostic antigen is produced on the recovered antigen complex common for histoplasmosis and coccidioidomycosis.;EFFECT: higher stability and indirect hemagglutination reproducibility.;2 cl, 3 ex, 2 tbl