Escherichia coli BL21(DE3)/pAFP11D3 STRAIN - PRODUCER OF 404 TO 609 AMINO ACID FRAGMENT OF HUMAN ALPHA-FETOPROTEIN

摘要

FIELD: medicine.;SUBSTANCE: invention refers to biotechnology, particularly to recombinant production of human oncofetal proteins, and can be used for producing a 404 to 609 amino acid fragment of human alpha-fetoprotein. The Escherichia coli BL21(DE3)/pAFP11D3 strain - a producer of the 404 to 609 amino acid fragment of human alpha-fetoprotein is produced by Escherichia coli cell transformation of the BL21(DE3) strain of plasmid DNA pAFP11D3.;EFFECT: invention allows reducing potential human viral contamination when using a recombinant method to produce said alpha-fetoprotein fragment exhibiting affinity to an alpha-fetoprotein receptor and exposed to receptor-mediated endocytosis.;2 cl, 7 dwg