METHOD FOR PRODUCING CULTURAL, POLYVALENT EPIDEMIC HAEMORRHAGIC FEVER DIAGNOSTICUM FOR INDIRECT IMMUNOFLUORESCENCE

摘要

FIELD: medicine.;SUBSTANCE: method for producing EHF Diagnosticum by reproduction of four hantaviruses - Puumala strain PUU-TDK/VERO, Dobrava strain DOB-EAT-VERO, Hantaan strain Ussuri-4590 and Seoul strain SK-515/Georgia-88, in a monolayer passaged cell culture of VERO-line grass monkey kidneys, followed by virus-specific antigen concentration test, polyvalent antigen production, antigen fixation on a slide and UV processing.;EFFECT: invention provides a more sensitive diagnostic test, presenting a low-price production process and more effective use of the EHF Diagnosticum.;4 cl, 2 ex, 1 tbl