METHOD OF OBTAINING BIOLOGICALLY ACTIVE SUBSTANCE FROM EMBRIONIC-EGG MASS FOR PREPARATION OF ANTI-BURN PLATE AND ANTI-FIRE PLATE ON ITS BASE

摘要

FIELD: medicine.;SUBSTANCE: non-infected fresh hen eggs are selected with their further incubating for 9 days. On 5, 6, 7 days hen egg is radiated for 30 s at frequency of modulation 9 Hz and radiation voltage 9 mW with further cooling of eggs for 6-7 days at temperature 2-4°C and homogenisation of eggs. Obtained homogenate is subjected to processing by ultrasound in ultrasonic dispersant. To homogenate, processed with ultrasound, added is mixture, containing 0.1% solution of sodium benzoate and 0.1% of solution of potassium sorbate in equal parts, in weight ratio 1:1 respectively with further ultramicrofiltration through filters with 0.2 mcm diameter, obtaining biologically active substance for embryonic-egg mass. Homogeneous mass is prepared in the following way. Medical gelatin is covered with decoction, containing calendula decoction and chamomile decoction in weight ratio 1:1, keep at temperature 20-25°C for 55-60 minutes with further placement of decoction with gelatin on water bath at temperature 65-70°C until complete dissolusion of gelatin is obtained. Into obtained decoction with gelatin added are amikacin, furacilline, anesthesin, amaranth oil, glycerol and brilliant green with further mixing in homogeniser for 4-5 min obtaining homogeneous mass. To obtained homogeneous mass biologically active substance from embryonic-egg mass is added in specified ratio and mixed in homogeniser for 5-7 min. Obtained mass is poured into special vessels and are placed in thermostat for 3.5-4 hours at temperature 37-39°C with further cooling for 1-2 days until plates solidify in form of plates.;EFFECT: inventions make it possible for injuries to regenerate quickly, increase immune reactivity of the organism and make it possible to simplify method of obtaining anti-burn plate.;2 cl, 6 dwg, 2 tbl, 5 ex