A process for the modulation of the gene expression by changing the cpg content

摘要

A method for the targeted modulation of the gene expression, comprising:(i) providing a target nucleic acid sequence to be expressed,(ii) modifying the target nucleic acid sequence, wherein the number of in the target nucleic acid sequence existing cpg - dinucleotides with the use of the degeneracy of the genetic code to increase the gene expression is increased or to the reduction of the gene expression is reduced,(iii) cloning of the so modified target nucleic acid sequence having a modified number of cpg - dinucleotides in a suitable expression vector in operative linkage with a suitable transcription control sequence,(iv) express the modified target nucleic acid sequence in a suitable expression system.