Extract of a fruit body derivative of Antrodia cinnamomea, useful for treating inflammation, obtained by providing a dried fruit body of Antrodia cinnamomea, and extracting with ethanol to obtain acetic acid extract

摘要

Extract of a fruit body derivative of Antrodia cinnamomeaobtained by providing a dried fruit body of Antrodia cinnamomea, extracting with ethanol at a specific temperature to obtain an acetic acid extract, concentrating the acetic acid extract to obtain a concentrated product, and separating the concentrated product with acetic acid ethyl ester and water to produce an acetic acid ethyl ester extract, is claimed. Independent claims are also included for: (1) Antrocamphin A for inhibiting the formation of nitric oxide and prostaglandin-E2; and (2) inhibiting the formation of pro-inflammatory molecules, comprising bringing the investigated object in contact with the extract or Antrocamphin A. ACTIVITY : Antiinflammatory. MECHANISM OF ACTION : Nitric oxide inhibitor; Prostaglandin-E2 inhibitor; Nitric oxide synthase suppressor; Cyclooxygenase-2 suppressor. The ability of the acetic acid extract (ACE 500) comprising lipopolysaccharide (5 g/ml) and the ACE (500 mg/kg) to inhibit the formation of nitric oxide was tested via injecting the ACE and lipopolysaccharide, respectively into mice (6). The result after anesthetizing with ether and decapitating the mice after 12 hours showed that the ACE 500 exhibited inhibition of the nitric oxide production of 8.79+- 0.11 compared to a negative reference group comprising lipopolysaccharide (5 g/ml), which exhibited the nitric oxide production of 37.79+- 1.87.