FLUORESCENT TEMPERATURE PROBE AND TEMPERATURE MEASURING INSTRUMENT USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a temperature measuring instrument capable of performing temperature measurement of the insides of an individual living being, tissue, cell and organelle in a cell, a water solution, or a solid body such as metal and plastic, and performing quantitative imaging of temperature without being affected by a proteinous fluorescent temperature probe suitable to imaging by spatial solution (1 μm) having a high absolute temperature distribution nor by fluctuations of probe concentration in a measuring object.;SOLUTION: A fluorescent temperature probe is configured by connecting a temperature sensitivity fluorescent protein in which relative fluorescence intensity greatly changes within the range of 20 to 50°C and a reference fluorescent substance in which a change in relative fluorescent intensity is small at a fixed ratio.;COPYRIGHT: (C)2012,JPO&INPIT