It includes the gene which the rearrangement vector and the intended protein which include the gene which cell outside production

摘要

Simultaneously character culturing the microbe and the aforementioned microbe which are converted with the rearrangement manifestation vector which includes the intended protein which this invention, the intended protein further in details, to the cell exine of colon bacillus origin protein F (OmpF) tries will relate to the method where it secretes produces in the cell culture solution, to secrete in the rearrangement manifestation vector and the cell culture solution which include the gene the intended protein it is something regarding the method where it secretes produces in the cell culture solution. According to this invention, it does not fuse the intended protein with the other protein and it is possible in pure state to make secrete in the cell culture solution, yield separation refinement of the intended protein is possible.