Anion exchange adsorption methods and anion exchanger

摘要

(57) A method for removing a substance that carries the [Abstract] negative charge is present aqueous liquid (I), the hydrophobic structural positive charge and structure (anion exchanger), and (i) liquid a matrix carrying a plurality of ligands, including, under conditions which bring about coupling between the substance and the ligand, the method comprising the step of desorbing the substance (ii) from the matrix phase, and contacting, (I), respectively the ligand plus spacer has the following formula - formula SP --- [1 -N ⁺_(R 2 _R 3 _{R 4) Ar-R],} (A) _[Ar-R 1 -N ⁺ represents a ligand, in this case, a) Ar is an aromatic ring, b) _{R 1} is [(L) _n R _{'1] m,} where-is _(R 2 _R 3 _{R 4)]} m and n is an integer selected from 1 or zero, the amino nitrogen, · L is a thioether sulfur or an ether oxygen, · R _'1 is a divalent linker group, 1) linear, branched cyclic hydrocarbon group or chain, 2) -C (= NH) -, are more selective, _{c) R 2-4} are selected from hydrogen and lower alkyl, (B) SP is, _Ar-R 1 ^{N +} ( and a spacer which provides carbon is directly bonded to R ₂ R ₃ R _4), nitrogen, oxygen or sulfur and (C) ---, spacers _(Ar-R 1 -N ⁺ (R 2 R ₃ In particular, the desorption (II) stage (ii) is, R and when the substance is a serine protease, and represents a bond to the matrix - indicates that replacing the hydrogen of R _{4), (D)} -C (= NH) is' -. anion exchanger having a time, is carried out by anion exchange conditions, a method (a) high breakthrough capacity, which is characterized in that, (b) Screening method and (c) desalting protocol are also described.