Method of screening for target protein ubiquitination by ubiquitin ligase

摘要

It is an object of the present invention is to develop an experimental system for screening proteins that are ubiquitinated by ubiquitin ligase, to identify the target protein ubiquitination by using the same. The yeast two hybrid screening system, instead of the F-box protein (1) full-length and deleted the extent that it can not be combined with Skp1 or whole, the F-box motif is believed to be the binding region with Skp1 By mutant is used as bait a mutant F-box protein, resolution of (2) the target protein is reduced further (for example, deletion mutants or temperature-sensitive mutant strain) are used, it is possible to stabilize the target protein, as a result It was found that it is possible to screen.