Pre-clinical method for monitoring the continuous change of the circulating breast cancer cells in mice

摘要

provides a system for Tracks (CellTracks) (registered trademark) system, performs counting of CTC in blood. The system performs the identification and quantification of CTC concentrated immunomagnetically epithelial cells, and fluorescently labeled cells. In part, the prediction of survival, time of disease progression, and the absolute number of CTC detected in the peripheral blood in case of having a tumor, is a factor in the response to treatment. By be monitored repeatedly CTC in animal models is not possible, preclinical studies of circulating tumor cells (CTC) has been limited. The present invention provides a method for counting the CTC in blood specimens using a protocol similar to the in vitro diagnostic systems for the determination of CTC in patients, were collected from live mice. Therefore, this technique can be adapted to continuously monitor the CTC in the mouse xenograft tumor model of human breast cancer.