Measuring method and the measurement reagent of the measuring object material in the sample, manner, and the non unique color development restrainer null which suppress non unique color development

摘要

PROBLEM TO BE SOLVED: To provide a measuring method and a measuring reagent capable of accurately measuring a concentration of an object substance to be measured in a sample by inhibiting influences of various interfering substances such as hemoglobin, ascorbic acid and the like contained in the sample, in measuring the object substance to be measured in the sample by using a tetrazolium compound.;SOLUTION: In measuring the object substance to be measured in the sample by using the tetrazolium compound, the method and the reagent for measuring the object substance to be measured in the sample are characterized in that at least one selected from a group consisting of Trinder's reagents, superoxide dismutase and 1,2-dihydroxy-3,5-benzenedisulfonic acid or its salt is put or contained in a measurement reaction liquid or the measuring reagent, as a nonspecific coloring inhibitor for the tetrazolium compound.;COPYRIGHT: (C)2008,JPO&INPIT