Antibodies directed against the Marburg I polymorphism of factor VII-activating protease (FSAP) and their production and use

摘要

The antibodies raised against the variant Marburg I factor VII-activating protease (FSAP) that bond specifically at the FSAP MRI variant but not at the FSAP wild type protein or other mutant variations which are at other positions of the FSAP protein through amino acid exchange, are new. The antibodies are produced using a peptide with 5-20 amino acids and preferably 10-15 amino acids. Antibodies raised against the variant Marburg I factor VII-activating protease (FSAP) that bond specifically at the FSAP MRI variant but not at the FSAP wild type protein or other mutant variations which are at other positions of the FSAP protein through amino acid exchange, are new. The antibodies are produced using a peptide with 5-20 amino acids and preferably 10-15 amino acids, where the peptides comprise peptide (I) comprising 10-15 amino acids that contain the amino acid sequence Glu-Cys-Glu-Lys-Arg (SEQ ID NO. 1) Independent claims are also included for the following: (A) reagent containing one or more peptides and/or one or more antibodies as above; (B) assay kit containing the reagent; (C) animal-, plant-, fungal-, prokaryotic or isolated human cells producing antibodies as above; (D) hybridoma cell lines from the cells in (C) deposited at the DSMZ under the number DSM ACC2675, DSM ACC2676, DSM ACC2674, DSM ACC2725 and DSM ACC2726; (E) quantitative or qualitative detection of Marburg I variant of FSAP in a sample using one or more (I) and/or one or more antibodies as above. ACTIVITY : Virucide. MECHANISM OF ACTION : Antibody Therapy.