Method for producing single cell liposomes by a two-stage emulsification method using an external aqueous phase containing a specific dispersant, and a method for producing a single cell liposome dispersion or a dry powder thereof using the method for producing single cell liposomes

摘要

Disclosed is a method for producing liposomes, a liposome dispersion or a dry powder thereof by a two-stage emulsification method using an additive (dispersing agent) whereby a liposome dispersion and a dry powder thereof, which ensure the prevention of the leakage of an encapsulated drug or the like from liposomes during prolonged storage and can be stably used over a long period of time, can be obtained. A method for producing liposomes by a two-stage emulsification method, characterized by using, in the secondary emulsification step, an outer aqueous phase containing a dispersing agent (hereinafter referred to as "a specific dispersing agent") which forms no molecular self-assembly or exclusively forms molecular self-assemblies having a volume-average particle diameter equal to or less than 10 nm; and a method for producing a liposome dispersion and a dry powder thereof using the aforesaid production method. The aforesaid specific dispersing agent preferably contains at least one component selected from among, for example, gelatin, albumin, dextran and a polyalkylene oxide-based compound.