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Expression in specific genetic modifications of the trehalose-6-phosphate synthase activity and homology or atypia of the environment

机译:海藻糖-6-磷酸合酶活性和环境同源性或非典型性的特定基因修饰表达

摘要

(57) Abstract This invention, configuration of the TPS gene qualification uniquely, induce and/or shows organ unique revelation, the plant, it regards the preparation manner of the eucaryote which is chosen from the animal and Eumycetes etc. This manner includes the following process: The TPS gene is prepared; The appropriate qualification for the TPS gene, the gene is designed balance by lining up in the corresponding gene of the yeast, it verifies whether and some part of the gene exceeds 5 ' the end of the yeast gene and extends; In order to achieve the increase of toreharosu 6 phosphate synthetase activity, exceeding 5 ' the end of the yeast gene, the part of the N end field of the TPS gene which it extends, desirably lack or non- to life it converts the complete extension part; The qualification gene in the manifestation vector configuration and induce and/or cloning is done under controlling the organ unique promotor; Having with the manifestation vector which you obtain, character it converts the cell or system of the plant; Character regeneration obtain the cell or system of the plant which is converted.
机译:(57)<摘要>本发明独特地具有TPS基因鉴定的构型,诱导和/或显示器官独特的揭示,对于植物而言,它涉及选自动物和真真菌等的真核生物的制备方法。制备TPS基因;对TPS基因的适当鉴定,通过排列在酵母的相应基因中来平衡该基因的设计,验证该基因的一部分是否超过酵母基因的5'末端并延伸;为了实现toreharosu 6磷酸合成酶活性的增加,超过酵母基因的5'末端,它延伸的TPS基因N末端字段的一部分,理想的是缺乏或不生命,它将完全延伸部分;在控制载体独特启动子的情况下完成表现载体构型以及诱导和/或克隆的鉴定基因。具有获得的表现载体,将其转化为植物的细胞或系统;角色再生获得被转化的植物的细胞或系统。

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