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CHIMERAL INTERNAL RIBOSOMAL ENTRY SITE SEQUENCE AND USES THEREOF
CHIMERAL INTERNAL RIBOSOMAL ENTRY SITE SEQUENCE AND USES THEREOF
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机译:嵌合内部核糖体进入位点序列及其用途
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摘要
An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of a Rhopalosiphum padi virus (RhPV) IRES sequence at least 90% identical to SEQ ID NO: 2; and a second nucleic acid operable linked to the portion of the RhPV IRES sequence for expressing a second protein in the mammalian or insect host cells.
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机译:公开了一种能够在哺乳动物或昆虫宿主细胞中表达基因的改良的杆状病毒载体及其用途。改进的杆状病毒载体依次包括:启动子;和与所述启动子可操作地连接的第一核酸,用于在哺乳动物或昆虫宿主细胞中表达第一蛋白;嵌合体内部核糖体进入位点(IRES),其中包括与SEQ ID NO:1至少90%相同的一部分肠病毒(EV)IRES序列和一部分 Rhopalosiphum padi I>病毒(RhPV)IRES与SEQ ID NO:2至少90%相同的序列;第二核酸可操作地连接至RhPV IRES序列的一部分,以在哺乳动物或昆虫宿主细胞中表达第二蛋白质。
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