LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING HYBRIDIZATION CHAMBER ARRAY AND NEGATIVE CONTROL CHAMBER WITHOUT PROBES

摘要

A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber without the ECL probes.