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Gene-transfer vector comprising helper-component protease gene of papaya ringspot virus for broad-spectrum virus resistance in crops and use thereof
Gene-transfer vector comprising helper-component protease gene of papaya ringspot virus for broad-spectrum virus resistance in crops and use thereof
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机译:包含番木瓜环斑病毒辅助成分蛋白酶基因的农作物广谱病毒抗性基因转移载体及其应用
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摘要
Provided is a recombinant plasmid having a control sequence and a coding sequence fragment of Papaya ringspot virus (PRSV) helper-component protease gene (HC-Pro gene) operably linked to the control sequence. A recombinant microorganism derived therefrom is also provided. A method for providing plants with resistance against virus is also provided. Use of PRSV HC-Pro gene or fragment thereof in generating plants with resistance against virus is also provided. It is proven that the PRSV HC-Pro transgenic plants can solve the problem resulting from breakdown by gene silencing suppression and provide broad-spectrum resistance to various PRSV strains of different geographical origins.
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