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Polymerase Enhancing Factor (PEF) Extracts PEF Protein Complexes Isolated PEF Proteins and Methods for Purifying and Identifying
Polymerase Enhancing Factor (PEF) Extracts PEF Protein Complexes Isolated PEF Proteins and Methods for Purifying and Identifying
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机译:聚合酶增强因子(PEF)提取PEF蛋白复合物分离的PEF蛋白及其纯化和鉴定方法
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摘要
The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for a Pyrococcus furiosus PEF (P45), which was used to produce a recombinant PEF.
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