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Eukaryotic expression system for the incorporation of stable isotopes into proteins
Eukaryotic expression system for the incorporation of stable isotopes into proteins
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机译:用于将稳定同位素掺入蛋白质的真核表达系统
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摘要
Methods for producing stable isotope-labeled recombinant protein are provided. The methods include isolating a stable isotope-labeled recombinant protein from a Trichoplusia ni larva expressing a recombinant protein, which Trichoplusia ni larva has ingested a food source comprising stable isotope-labeled algae, thereby resulting in incorporation of a stable isotope into the recombinant protein to produce the stable isotope-labeled recombinant protein.
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机译:提供了产生稳定的同位素标记的重组蛋白的方法。这些方法包括从表达重组蛋白的 Trichoplusia ni I>幼虫中分离出稳定的同位素标记的重组蛋白,而 Trichoplusia ni I>幼虫已经摄取了包含稳定同位素标记的藻类的食物。 ,从而导致将稳定的同位素掺入重组蛋白中以产生稳定的同位素标记的重组蛋白。
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