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Methods for stratification and storage of somatic embryos
Methods for stratification and storage of somatic embryos
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机译:体细胞胚的分层和贮藏方法
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摘要
In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week to produce stratified cotyledonary somatic embryos.
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