Primers, Probes, Methods and Kits for the detection of eml4-alk variants

摘要

Primers, probes and sets of elements (kits) for the detection of sequences of eml4-alk variants in a Sample and detection methods of eml4-alk variants using the primers, probes and Joint elements are disclosed.Claim 1: a method to detect the presence of a variant of eml4-alk in a Sample, wherein Said method comprises: (a) to isolate a Nucleic Acid of the sample, where the sample includes DNA sequences; (b) conduct an Amplification reaction of these STRI ng + Garter Set This DNA in the sample,Where such Amplification reaction comprises a First primer capable of specifically aligned with a sequence of a variant of eml4-alk in first position in a DNA sequence of eml4-alk, wherein Said First primer is selected from the group consisting of the SEQ ID no: 1, the SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 7The SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: 18, SEQ ID no: 19, SEQ ID no: 1, SEQ ID no: 1, SEQ ID no: SEQ ID no: 22, 23, 24 and the SEQ ID no: SEQ ID no: 25 and a Second Primer capable of specifically aligned in a second position in a DNA sequence of eml4-alk,Where the first and second primers are aligned with different Chains of DNA double chain eml4-alk,Where the Amplification reaction has the ability to produce a specific Amplification Product Variant eml4-alk when Sample DNA sequences comprising a DNA sequence of eml4-alk comprising a sequence of a Variant in the first position of the DNA sequence of eml4-alk; and (c) show the Amplification Products produced by the REaccion Amplification, where the detection of a specific Amplification Product Variant eml4-alk variants is a positive indicator of eml4-alk in this sample.