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Preparation method of biodegradable Membranes for Soft Tissue Regeneration.
Preparation method of biodegradable Membranes for Soft Tissue Regeneration.
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机译:用于软组织再生的可生物降解膜的制备方法。
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摘要
The present Invention is a method that is mechanically and Chemically in the small intestine of warm blooded vertebrate to withdraw the tunics serosa and mucosa, Muscle, and obtain the tunica submucosa as a remnant.In summary, the portion of the jejunum of the small intestine of warm blooded vertebrate freshly slaughtered and stored at 4oC in 0.9% saline solution. Subsequently scraped in longitudinal direction to make an initial clearance of the tunica mucosa and washed to be clean.Cut the small intestine in longitudinal direction, and both sides are scraped to remove the remaining tunica mucosa and muscularis and serosa layers to obtain a White Translucent membrane. Washed by immersing in distilled Water and 0.9% Saline for 30 - 120 minutes until the waste Blood, stirring constantly and changing the water every 20 minutes.Scrape again to remove the last Remnants of the tunics serosa, muscularis and mucosa. Exposed to a mixture of 0.5% sodium hypochlorite and Hydrogen peroxide by 0.5% 15 - 40 minutes to remove cells remaining, stirring constantly and noting that no structural changes are noticeable.Washed with phosphate Buffer Solution, stirring constantly for 15 minutes and then washed with distilled Water and 0.9% saline solution. The Membrane is obtained according to the application; The Membranes can be used individually or form an aggregate of several overlapping Membranes according to the desired resistance to particular forms and in addition to the Membranes.Once given the way, dehydrate formed Membranes exposed to laminar flow sterile Air. The whole process must be carried out in maximum conditions of Asepsis at room temperature. The Membranes must be sterilized before being implanted in a living being.Applications include the use of Membranes, maxillofacial surgery, Plastic and reconstructive surgery, cardiovascular surgery, Gastroenterology and Urology.
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