METHOD FOR PREPARING BIODEGRADABLE MEMBRANES FOR SOFT TISSUE REGENERATION

摘要

The invention relates to a method comprising the mechanical and chemical treatment of the small intestine of a warm-blooded vertebrate, in order to remove the mucous, serous and muscular coats and to obtain the remaining submucous coat. In short, the jejunum portion of the small intestine is obtained from a recently deceased warm-blooded vertebrate and is stored at 4°C in 0.9% saline solution. Subsequently, longitudinal scraping is performed for the initial removal of the mucous coat and it is then washed until clean. The small intestine is cut longitudinally and both surfaces are scraped in order to remove the remaining mucous coat and the muscular and serous coats, until a translucent white membrane is obtained. This is then submerged in 0.9% saline solution or distilled water and washed for 30 to 120 minutes until the blood residues disappear, constantly stirring same and changing the water every 20 minutes. Scraping is performed once again in order to eliminate the last remanents of the serous, muscular and mucous coats. It is then exposed to a mixture comprising 0.5% sodium hypochlorite and 0.5% hydrogen peroxide for 15 to 40 minutes in order to eliminate the remaining cells, constantly stirring same and observing that no obvious structural changes occur. It is washed with a phosphate buffer solution for 15 minutes, stirring constantly, and subsequently washed with 0.9% saline solution or distilled water. The membrane obtained is shaped according to use. The membranes can be used individually or to form an aggregate of multiple stacked membranes depending on the desired strength and the membranes can be given particular shapes. Following shaping, the membranes are dehydrated by means of exposure to a laminar sterile air flow. The entire method must be carried out in maximum asepsis conditions at ambient temperature. The membranes must be sterilised prior to implantation in a living organism. Said membranes can be used in maxillofacial surgery, reconstructive and plastic surgery, cardiovascular surgery, gastroenterology and urology, inter alia.