Method for accessing specific pyrimidine nucleosides in a structure of two-chain DNA for reacting with antibodies, which react with such nucleosides

摘要

the solution describes the way zpru00edstupnenu00ed pyrimidine nucleoside in strukture dvouretezcovu00e9 dna for reaction with antibodies which react with these nucleosides.this method is in the incubation dvouretezcovu00e9 dna in presence of monobasic ion copper and oxygen and / or active okyslicovadel.the source of these ion may be aqueous solutions containing ions, copper, or can act on jednomocnou honey, which was on the dna obtained. volitelne apply wash out substance.this step leads to undesirable substances produced during the incubation of the obstacle in the solution with ions jednomocnu00e9 medi.peace zpru00edstupnenu00ed can be increased by subsequent incubation of dna with the enzyme in the exonukleu00e1zovou activity.dna is also possible first incubated in solution with dvojmocnu00fdmi ions medi.the figure below turn in jednomocnu00e9 ions in solution with produced by incubation in the presence of oxygen to cause substances and then u0161tepenu00ed dna.in this case, the degree zpru00edstupnenu00ed is generally lower than in solutions containing monobasic ion and requires the use of zpru00edstupnenu00ed using an enzyme with exonukleu00e1zovou activity.