Hybridization probe and primers for detecting expression of porcine cystathionine-gamma-lyase

• 1. 猪圆环病毒2型和猪圆环病毒4型双重实时荧光定量PCR检测用引物、探针及检测方法 [P] . 中国专利： CN112410466A . 2021-02-26
• 2. 副猪嗜血杆菌和猪支气管败血波氏杆菌双重荧光定量PCR检测的引物、探针及方法 [P] . 中国专利： CN110951902A . 2020-04-03
• 3. Hybridization probe and primers for detecting expression of porcine 3-mercaptipyruvate-sulfurtransferase [P] . CZ23221U1 . 2012-01-18

  机译： 用于检测猪3-巯基丙酮酸硫转移酶表达的杂交探针和引物

• 4. Hybridization probe and primers for detecting expression of porcine cystathionine-beta-lyase [P] . CZ23224U1 . 2012-01-18

  机译： 猪胱硫醚β-裂解酶表达的杂交探针和引物

• 5. Hybridization probe and primers for detecting expression of swine calcineurin B [P] . CZ21223U1 . 2010-09-08

  机译： 用于检测猪钙调神经磷酸酶B表达的杂交探针和引物

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• 11. A relative quantitative method to detect OCT4A gene expression by exon-junction primer and locked nucleic acid-modified probe* [O] . Ren, Jian-jun, Meng, Xing-kai 2011
• 12. Method to Detect Differential Gene Expression in Cross-species Hybridization Experiments at Gene and Probe Level. [R] . Chakicherla, A., Felton, J., Rocke, D., 2009

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 应用PCR法制备地高辛标记探针原位杂交检测绵羊卵巢褪黑素受体Mel1aα基因表达 [C] . 张勇 ,朱家桥 ,李发弟 . 中国畜牧兽医学会2010学术年会暨第二届中国兽医临床大会 . 2010
• 3. 大白猪×梅山猪杂交组合和长白猪×大白猪杂交组合亲子代肌肉组织的基因差异表达研究 [A] . 刘永刚 . 2006