There are provided a fuel cell and a production method therefor in which one or more types of enzymes or further coenzymes are enclosed in a micro space so that electrons can be efficiently extracted from a fuel such as glucose or the like by an enzyme reaction using the micro space as a reaction field, thereby producing electric energy, and in which the enzyme or further the coenzyme can be easily immobilized on an electrode. Enzymes 13 and 14 and a coenzyme 15 necessary for an enzyme reaction are enclosed in liposome 12, and the liposome 12 is immobilized on a surface of an electrode composed of porous carbon or the like to form an enzyme-immobilized electrode. An antibiotic 16 is bonded to a bimolecular lipid membrane constituting the liposome 12 to form one or more pores 17 permeable to glucose. The enzyme-immobilized electrode is used as, for example, a negative electrode of a biofuel cell.
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