Bacterial adherence and adherence to mucus, epithelial cells and other cells.

摘要

Refers to a Kit of Enzyme Linked Immunosorbent Assay (ELISA) for non Radioactive test bacterial adherence and adherence to Mucus and epithelial cells, which includes: (i) a Solid support which is coated with Mucus and epithelial cells on the same As mucus, the mucus of Pig proximal ileum, duodenum, ChickenAmong others; (ii) a sample comprising bacteria such as E. coli; (iii) a primary antibody specific for these bacteria is a polyclonal Antibody conjugated with Horseradish Peroxidase (HRP) specific for antigens or serotypes of E and K.Coli; (iv) a labeled Secondary Antibody is detectable as Pig immunoglobulin IgG coupled to peroxidase, specific for the primary Antibody; and (V) a substrate that allows the visualization of Secondary Antibody that is a colorimetric composition such as 3,3 ', 5,5' - Tetrametilbencidina